Superdex 75 10/300 GL Prepacked column for smallscale preparative purification (µg to mg) or characterization of proteins in the molecular range 3000 to 70 000, like recombinant, tagged proteinsSuperdex prepacked columns are designed for highperformance, laboratoryscale separations of proteins, peptides, and other biomolecules according to sizeSuperdex 0 Increase 5/150 GL 5 x 150 3 4 to 50 42 000 045 075 50 Superdex 0 Increase 32/300 32 x 300 24 4 to 50 48 000 0075 015 45 Table 3 Column choice for different applications Type of application/column 10/300 GL 5/150 GL 32/300 Smallscale preparative runsSuperdex 75 Increase 32/300, Superdex 75 Increase 5/150 GL, and Superdex 75 Increase 10/300 GL columns Fig 2 Elution volumes for globular proteins of various molecular weights on Superdex 75 Increase 10/300 (blue), Superdex 0 Increase 10/300 (green) , and Superose 6 Increase 10/300 (orange) Note!
Chromatographic Separation And Calibration Curve Of Standard Proteins Download Scientific Diagram
Superdex 75 16/60 calibration
Superdex 75 16/60 calibration-Load 16/60 Superdex 75 (prep grade) column at an elution rate of 1 ml/min The molecular weights of the proteins were estimated from the elution profile calibrated with the LMW Gel Filtration Calibration Kit (Amersham, UK) Figure 1 (a) SARSCoV N protein fragments studied in this paper Designations of the fragments are listed on the left (b)16/600 and 26/600 Superdex 75 prep grade, and HiLoad 16/600 and 26/600 Superdex 0 prep grade (pg) are prepacked XK columns designed for preparative size exclusion chromatography Superdex prep grade is a composite matrix of dextran and crosslinked agarose The steep selectivity of dextran and the high
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The soluble and refolded protein was loaded onto a HiLoad Superdex 75 prep grade (16/60) column, equilibrated with 50 mM sodium phosphate (pH 52), 5 mM EDTA As a reference, a low molecular weight gel filtration calibration kit was used conalbumin (75 kDa), ovalbumin (43 kDa), carbonic anhydrase (29 kDa), ribonuclease A (137 kDa) andSuperdex ® 75 10/300 GL is a prepacked gel filtration column for highresolution, semipreparative, and analytical separations of biomolecules Superdex ® 75 has a separation range for molecules with molecular weights between 3 000 and 70 000 Superdex ® prepacked columns are designed for highperformance, laboratoryscale separations of proteins, peptides, and otherProteins on HiLoad 16/60 Superdex 75 pg column Fig 7 Chromatographic separation and calibration curve for the standard proteins on HiLoad™ 16/60 Superdex 0 pg column Fig 9 Chromatographic separation and calibration curve for the standard proteins on HiPrep™ 16/60 Sephacryl S300 HR column
0 40 60 80 100 1 ferritin aldolase conalbumin ovalbumin carbonic anhydrase ribonuclease A aprotinin Ve Column HiLoad 16/60 Superdex 0 pg Geometric column volume (V C) 1 mL Sample volume 500 µL Buffer 50 mM phosphate, 150 mM NaCl, pH 72 Flow rate 1 mL/min System ÄKTAexplorer 10 Detection Absorbance at 280 nm Volume (mL)Superdex gel filtration media are listed in Appendix C, Table 1 With their high physical and chemical stability, very high batchtobatch reproducibility, and Regulatory Support File backup, Superdex 30 prep grade, Superdex 75 prep grade and Superdex 0 prep grade are ideal for all stages of an industrial scale operation from research andHiLoad ® 16/600 Superdex ® 0 prep grade are prepacked XK columns designed for highresolution preparative gel filtration chromatography The column is an excellent choice for separating MAbs from dimers and contaminants Superdex ® prep grade (pg) is produced by covalent bonding of dextran to highly crosslinked agaroseThe separation properties of these
This Amersham (GE Healthcare) SuperDex 75 Prep Column is new from surplus stock It ships in the original packaging It comes with the instruction sheet and XK16 Accessory Kit () The column is labeled XK 16 HiLoad 16/60 Superdex 75 Prep Grade Part Number More information can be found on the Amersham/GE websiteThe dimer/monomer ratios for the concentrations of 1, 2, and 7 mg/ml are 065, 066, and 080, respectively In the SECFPLC experiment, each protein sample was loaded to a HiLoad 16/60 Superdex 75 prep grade column preequilibrated with the buffer and then eluted at a flow rate of 1 ml/min with a detection of absorbance at 280 nmSuperdex 0 Increase 10/300 GL Prepacked Column (1) Superdex 0 Increase 5/150 GL (1) Superdex 0 Prep Grade (1) Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column (1) Superdex 30 Prep Grade, 150 mL (1) Superdex 75 Prep Grade (1) Superose 12 Prep Grade, 125 mL (1) Superose 6 Prep Grade, 125 mL (1)
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Find SigmaAldrichU MSDS, related peerreviewed papers, technical documents, similar products & more at SigmaAldrichSuperdex® Gel Filtration Column phase HiLoad 16/60 Superdex 75 PG, L × ID 60 cm × 16 mm, 35 μm particle size;Superdex 75 Increase 32/300, Superdex 75 Increase 5/150 GL, and Superdex 75 Increase 10/300 GL columns Selectivity of the resin Superdex 75 Increase and the related Superdex 0 Increase and Superose™ 6 Increase belong to the new generation of SEC resins based on highflow agarose with small beads The
Hiload 16 60 Superdex 0 Prep Grade Column Ge Healthcare Bioz Ratings For Life Science Research
Superdex 75 16 60 Ge Healthcare Bioz Ratings For Life Science Research
Can be used with standalone pump or chromatography system High resolution – for high purity Scalable – separations can be scaledSuperdex 75 10/300 GL and Superdex 0 10/300 GL are Tricorn™ high performance columns The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, DNA fragments (HiLoad 16/60 or 26/60 Superdex 75 prep grade HiLoad 16/60 or 26/60 Superdex 0 prep grade Dismantling tool, Support screens, net ringsμm), Oring, domed nuts Equilibration before a new run Regenerate the column after each run with one column volume of buffer at 30 cm/h (1 ml/min for XK 16/60 or 26 ml/min forXK 26/60)
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Native molecular mass of the purified enzyme was estimated by using size exclusion chromatography on a calibrated Superdex 75 (16 × 60 cm) column The column was equilibrated with mM phosphate buffer (pH 74) containing 300 mM NaClHiLoad 16/600 and 26/600 Superdex 30 prep grade, HiLoad 16/600 and 26/600 Superdex 75 prep grade, and HiLoad 16/600 and 26/600 Superdex 0 prep grade (pg) are prepacked XK columns designed for preparative gel filtration Superdex prep grade is a composite matr ix of dextran and highly crosslinkedChromatographic separation and calibration curve for the standard proteins on Superdex 0 10/300 GL column 0 100 0 300 400 mAU 0 5 10 15 25 ml 000 0 040 060 080 100 K av 010 030 050 070 090 Conalbumin Ovalbumin Carb anh RNase A Aprotinin C O CA R Apr M r logarithmic scale 10 3 10 4 10 5 10 6 FigA102 Chromatographic
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Find SigmaAldrichGE MSDS, related peerreviewed papers, technical documents, similar products & more at SigmaAldrichCalibration Superdex 75 Increase 10/300 GL 3 × 10to 7 × 103 4 24 100 Superdex 75 Increase 5/150 3 × 10to 7 × 103 4 3 125 HiPrep 16/60 Sephacryl S0 HR 5 × 10to 25 × 103 5 1 500 HiPrep 26/60 Sephacryl S0 HR 5 × 10to 25 × 103 5 3 1000Journal bioRxiv Article Title Structural studies of thyroid peroxidase show the monomer interacting with autoantibodies in thyroid autoimmune disease doi /8767 Article Snippet As a final polishing step, ΔproTPOe was further purified by a HiLoad Superdex S0 16/60 column (GE Healthcare) using gel filtration buffer (1x PBS pH 74, 001% Tween )
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Superdex 75 (16/60) and HR10/30 prepacked columns, OctylSepharose Fast Flow media, SPSepharose High Performance media, gel filtration low molecular weight (LMW) calibration kit, and an isoelectric focusing (IEF) calibration kit were purchased from GE life sciences (USA) SEC isolation of peptide conformers The oxidized AβS26C was subjected to SEC on a preparative HiLoad 16/60 Superdex 75 column (GE Healthcare) eluted with 25 m m g for min and ml of supernatant injected onto the column Peptides were detected by absorbance at 280 nm and 12 ml fractions collected DEAESepharose FF, HiLoad 16/60 Superdex 0, HiLoad 16/60 Superdex 75 and PD10 columns, and the Broad pI calibration kit (pI 30–100) were purchased from GE Healthcare Co (USA) Other reagents were special grade and were purchased commercially 22 Purification of fibrinolytic enzyme
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Fig 8 Chromatographic separation and calibration curve for the standard proteins on HiLoad 16/60 Superdex 75 pg column Fig 9 Chromatographic separation and calibration curve for the standard proteins on HiPrep 16/60 Sephacryl S300 HR column Note Aprotinin may be excluded from the calculation of Kav due toSuperdex 75 Increase 10/300 GL is suitable for small scale preparative purification (μgmg) as a final polishing step, as well as for protein analysis and characterization The columns are supplied with two fingertight connectors 1/16" male for connection to ÄKTA ™ or other systems Table 1Resin data Matrix Composite of crosslinkedHiPrep™ 16/60 Sephacryl® S0 HR Cytiva , column L × ID 60 cm × 16 mm, 50 μm avg part size;
70以上 Superdex 75 16 60 Superdex 75 16 60 Pressure Limit Saesipapictzzr
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Superdex 75 Increase 10/300 GL, 5/150 GL, and 32/300 are prepacked columns for versatile use in size exclusion chromatography applications The columns are intended for use in preparative purification as well as for characterization and analysis of proteins with molecular weights between M r 3000 and 70 000, such as recombinant tagged proteinsGE Healthcare hiload 16 60 superdex 75 prep grade column Hiload 16 60 Superdex 75 Prep Grade Column, supplied by GE Healthcare, used in various techniques Bioz Stars score 99/100, based on 1 PubMed citations ZERO BIAS scores, article reviews, protocol conditions and more During purification the Histagged IBD protein eluted from the HiLoad Superdex 75 pg 16/60 column at 7805 ml, suggesting that it is a monomer in solution when compared with calibration standards (GE Healthcare) In the preparation of IBD for analytical centrifugation, pRK172HisTEVIBD was transformed into E coli C41 (D) cells
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Gel filtration is performed at 4 °C on a Superdex 75 (26/60) (CABM Rutgers) or Superdex 75 Hiload 16/60 (U of Toronto) equilibrated with the corresponding NMR buffer at a flow rate of 25 ml/min The column is calibrated with LMW gel filtration calibration kit () from GE Healthcare A calibration curve is prepared by measuring theCalibration curves using prepacked Superdex Increase, Superose Increase, and Sephacryl columns are shown in Figures 2 to 11 proteins on HiPrep 16/60 Sephacryl S0 HR column Fig 11 Chromatographic separation and calibration curve for theHiLoad Superdex 0 preparation grade resin is well suited for rapid, preparative protein purification and scaleup Its selectivity curve gives a broad fractionation range from M r ~ 10 000 to 600 000, with excellent resolving power for antibody monomers, dimers and contaminants of lower molecular weight, such as albumin and transferrin Read more
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For example I have a 14 kDa protein that elutes after 130 ml on a Superdex 75 16/60 (ie after the column volume)!Histagged IBD protein eluted from the HiLoad Superdex 75 pg 16/60 column at 7805 ml, suggesting that it is a monomer in solution when compared with calibration standards (GE Healthcare) In the preparation of IBD for analytical centrifugation, pRK172HisTEVIBD was transformed into E coli C41 (D) cellsJected to SEC on a preparative HiLoad 16/60 Superdex 75 column (GE Healthcare)elutedwith25m M ammoniumacetate,pH85,ataflowrate of 06 ml/min using an AKTA FPLC system (GE Healthcare)
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Superdex 0 10/300 GL 1 × 104 to 6 × 105 24 100 Superdex 0 5/150 1 × 104 to 6 × 105 3 125 Superdex 0 PC 32/30 1 × 104 to 6 × 105 24 10 Superdex prep grade – HiLoad™ prepacked columns HiLoad 16/60 Superdex 75 pg 3 × 103 to 7 × 104 1 500 HiLoad 26/60 Superdex 75 pg 3 × 103 to 7 × 104 3 1000I wouldn't worry too much about your apparent MW being 30 kDa and not 40 TheLow molecular weight range from M r 6500 to 75 000 for LMW Kit High molecular weight range from M r 43 000 to 669 000 for HMW Kit The kits are suitable for calibration of highresolution SEC resins and columns such as Superdex, Superose, and Sephacryl
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GE Healthcare hiload 16 60 superdex 75 prep grade size exclusion chromatography column superdex Hiload 16 60 Superdex 75 Prep Grade Size Exclusion Chromatography Column Superdex, supplied by GE Healthcare, used in various techniques Bioz Stars score 99/100, based on 1 PubMed citations ZERO BIAS scores, article reviews, protocol conditionsThe whole fractionation rangeCalibration standard for gel filtration/size exclusion chromatography (SEC) columns used in protein purification and analysis under nondenaturing conditions It is appropriate for gels with exclusion limits approximately 60,000 to 5,000,000 Da The mixture contains thyroglobulin, gglobulin, ovalbumin, myoglobin, and vitamin B12
Superdex 75 Increase Ge Healthcare Bioz Ratings For Life Science Research
Superdex 75 Increase Ge Healthcare Bioz Ratings For Life Science Research
HiLoad ® 16/600 Superdex ® 75 prep grade are prepacked 1 mL XK columns designed for highresolution preparative gel filtration chromatography separating proteins and polynucleotides Superdex ® prep grade (pg) is produced by covalent bonding of dextran to highly crosslinked agaroseThe separation properties of these media are determined by the dextran componentElution profile of the purified Sumofused DGKαCD from Superdex 75 16/60 column Void volume (V 0) and the elution volume of the standard molecules, γglobulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), and vitamin B12 (13 kDa) are indicated by arrows Most of proteins eluted in a void volume of the column (43 mL)Ligation is carried out at varied temperatures like 16, 22, 25, 37 degrees and for different time like 16 hrs, overnight, 46 hours, 2 hrs, 10 mins What among all these would work best for
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Gel Filtration Calibration Kits
The dimer/monomer ratios for the concentrations of 1, 2, and 7 mg/ml are 065, 066, and 080, respectively In the SECFPLC experiment, each protein sample was loaded to a HiLoad 16/60 Superdex 75 prep grade column preequilibrated with the buffer and then eluted at a flow rate of 1 ml/min with a detection of absorbance at 280 nmSuperdex 0 Increase 32/300 4 to 50 µL 0075 mL/min >48,000 Highresolution analysis and purification of small sample volumes, especially when sample amount is limited and low buffer consumption is important Superdex 0 Increase 10/300 GL 25 to 500 µL 075 mL/min > 48,000HiLoad Superdex 75 pg prepacked columns are for highresolution size exclusion chromatography of recombinant proteins from samples up to 5 mL or 13 mL Prepacked format – for convenience and reproducibility;
Calibration Curve Of Kav For The Standard Proteins On Hiload 16 60 Download Scientific Diagram
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Superdex prep grade (pg) is produced by covalent bonding of dextran to highly crosslinked agarose Dextran component determined the separation properties of these media Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range Mr 10 000 600 000 (Superdex 0 pg)
Calibration Curve Of Kav For The Standard Proteins On Hiload 16 60 Download Scientific Diagram
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Calibration Curve Of Kav For The Standard Proteins On Hiload 16 60 Download Scientific Diagram
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